APPENDIX 1B Optimization of Transfection

  1. John K. Rose

Published Online: 1 MAY 2001

DOI: 10.1002/0471142301.nsa01bs01

Current Protocols in Neuroscience

Current Protocols in Neuroscience

How to Cite

Rose, J. K. 2001. Optimization of Transfection. Current Protocols in Neuroscience. 1:1B:A.1B.1–A.1B.3.

Author Information

  1. Yale University School of Medicine, New Haven, Connecticut

Publication History

  1. Published Online: 1 MAY 2001
  2. Published Print: NOV 1997


The single most important factor in optimizing transfection efficiency is selecting the proper transfection protocol. This overview discusses the most commonly used procedures: calcium phosphate-mediated gene transfer, DEAE-dextran-mediated gene transfer, electroporation, and liposome-mediated transfection. Fusion techniques such as protoplast fusion and microinjection may also be considered.