Appendix

APPENDIX 1H Preparation of Genomic DNA from Mammalian Tissue

  1. William M. Strauss

Published Online: 1 MAY 2001

DOI: 10.1002/0471142301.nsa01hs06

Current Protocols in Neuroscience

Current Protocols in Neuroscience

How to Cite

Strauss, W. M. 2001. Preparation of Genomic DNA from Mammalian Tissue. Current Protocols in Neuroscience. 6:1H:A.1H.1–A.1H.3.

Author Information

  1. Harvard Medical School and Beth Israel Deaconess Medical Center, Boston, Massachusetts

Publication History

  1. Published Online: 1 MAY 2001
  2. Published Print: FEB 1999

Abstract

There are a number of different procedures for the preparation of genomic DNA. They all start with some form of cell lysis, followed by deproteination and recovery of DNA. The main differences between various approaches lie in the extent of deproteination and in molecular weight of the DNA produced. The isolation procedure described here is relatively brief and relies on the powerful proteolytic activity of proteinase K combined with the denaturing ability of the ionic detergent SDS.