APPENDIX 1K Quantitation of DNA and RNA with Absorption and Fluorescence Spectroscopy
Published Online: 1 MAY 2001
Copyright © 2003 by John Wiley and Sons, Inc.
Lab Protocol Title
Current Protocols in Neuroscience
How to Cite
Gallagher, S. R. 2001. Quantitation of DNA and RNA with Absorption and Fluorescence Spectroscopy. Current Protocols in Neuroscience. 10:1K:A.1K.1–A.1K.8.
- Published Online: 1 MAY 2001
- Published Print: FEB 2000
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Reliable quantitation of nanogram and microgram amounts of DNA and RNA in solution is essential to researchers in molecular biology. In addition to the traditional absorbance measurements at 260 nm, two more sensitive fluorescence techniques are presented below. These three procedures cover a range from 5 to 10 ng/ml DNA to 50 mg/ml DNA.