Appendix

APPENDIX 1M Digestion of DNA with Restriction Endonucleases

  1. Kenneth D. Bloch

Published Online: 1 MAY 2001

DOI: 10.1002/0471142301.nsa01ms11

Current Protocols in Neuroscience

Current Protocols in Neuroscience

How to Cite

Bloch, K. D. 2001. Digestion of DNA with Restriction Endonucleases. Current Protocols in Neuroscience. 11:1M:A.1M.1–A.1M.12.

Author Information

  1. Massachusetts General Hospital, Charleston, Massachusetts

Publication History

  1. Published Online: 1 MAY 2001
  2. Published Print: MAY 2000

Abstract

Restriction endonucleases recognize short DNA sequences and cleave double-stranded DNA at specific sites within or adjacent to the recognition sequences. Restriction endonuclease cleavage of DNA into discrete fragments is one of the most basic procedures in molecular biology. This appendix describes restriction endonucleases and their properties.