APPENDIX 4H Euthanasia
Published Online: 1 NOV 2005
Copyright © 2005 by John Wiley & Sons, Inc.
Lab Protocol Title
Current Protocols in Neuroscience
How to Cite
Donovan, J. and Brown, P. 2005. Euthanasia. Current Protocols in Neuroscience. 33:4H:A.4H.1–A.4H.4.
- Published Online: 1 NOV 2005
- Published Print: OCT 2005
Euthanasia of laboratory animals must be performed by trained personnel using appropriate techniques, equipment, and reagents in order to effect a death that is humane and satisfies research requirements. Acceptable methods of euthanasia are painless or minimize distress, and are quick and easy to perform, safe for those performing the procedure, and efficient and economic. They are aesthetically acceptable and are done in the absence of other animals. In addition, these methods do not result in gross histological or histochemical changes that would adversely affect research results. This unit offers protocols for euthanasia employing carbon dioxide asphyxiation (see Basic Protocol 1), pentobarbital overdose (see Basic Protocol 2), exsanguination, and cervical dislocation for the mouse, rat, hamster, and rabbit.