UNIT 1.2 Enzymatic Synthesis of M1G-Deoxyribose
Published Online: 1 MAY 2001
Copyright © 2003 by John Wiley and Sons, Inc.
Lab Protocol Title
Current Protocols in Nucleic Acid Chemistry
How to Cite
Schnetz-Boutaud, N. C., Chapeau, M.-C. and Marnett, L. J. 2001. Enzymatic Synthesis of M1G-Deoxyribose. Current Protocols in Nucleic Acid Chemistry. 00:1.2:1.2.1–1.2.8.
- Published Online: 1 MAY 2001
- Published Print: FEB 2000
Adducts formed between electrophiles and nucleic acid bases are believed to play a key role in chemically induced mutations and cancer. M1G-dR is an endogenous exocyclic DNA adduct formed by the reaction of the dicarbonyl compound malondialdehyde with a dG residue in DNA. It is an intermediate in the synthesis of a class of modified oligodeoxyribonucleotides that are used to study the mutagenicity and repair of M1G. This unit presents methods for synthesizing M1G-dR by enzymatic coupling.