Unit

UNIT 1.4 Unnatural Nucleosides with Unusual Base Pairing Properties

  1. Donald E. Bergstrom

Published Online: 1 JUN 2009

DOI: 10.1002/0471142700.nc0104s37

Current Protocols in Nucleic Acid Chemistry

Current Protocols in Nucleic Acid Chemistry

How to Cite

Bergstrom, D. E. 2009. Unnatural Nucleosides with Unusual Base Pairing Properties. Current Protocols in Nucleic Acid Chemistry. 37:1.4:1.4.1–1.4.32.

Author Information

  1. Purdue University, West Lafayette, Indiana

Publication History

  1. Published Online: 1 JUN 2009
  2. Published Print: JUN 2009

Abstract

Synthetic modified nucleosides designed to pair in unusual ways with natural nucleobases have many potential applications in biology and biotechnology. This overview lays the foundation for future protocol units on synthesis and application of unnatural bases, with particular emphasis on unnatural base analogs that mimic natural bases in size, shape, and biochemical processing. Topics covered include base pairs with alternative H-bonding schemes, dimensionally expanded base pairs, hydrophobic base pairs, metal-ligated bases, degenerate bases, universal nucleosides, and triplex constituents. Curr. Protoc. Nucleic Acid Chem. 37:1.4.1-1.4.32. © 2009 by John Wiley & Sons, Inc.

Keywords:

  • nucleoside;
  • mimetic;
  • nonpolar;
  • degenerate;
  • universal