UNIT 1.4 Unnatural Nucleosides with Unusual Base Pairing Properties
Published Online: 1 JUN 2009
Copyright © 2009 by John Wiley & Sons, Inc.
Lab Protocol Title
Current Protocols in Nucleic Acid Chemistry
How to Cite
Bergstrom, D. E. 2009. Unnatural Nucleosides with Unusual Base Pairing Properties. Current Protocols in Nucleic Acid Chemistry. 37:1.4:1.4.1–1.4.32.
- Published Online: 1 JUN 2009
- Published Print: JUN 2009
Synthetic modified nucleosides designed to pair in unusual ways with natural nucleobases have many potential applications in biology and biotechnology. This overview lays the foundation for future protocol units on synthesis and application of unnatural bases, with particular emphasis on unnatural base analogs that mimic natural bases in size, shape, and biochemical processing. Topics covered include base pairs with alternative H-bonding schemes, dimensionally expanded base pairs, hydrophobic base pairs, metal-ligated bases, degenerate bases, universal nucleosides, and triplex constituents. Curr. Protoc. Nucleic Acid Chem. 37:1.4.1-1.4.32. © 2009 by John Wiley & Sons, Inc.