Unit

UNIT 1.5 Development of a Universal Nucleobase and Modified Nucleobases for Expanding the Genetic Code

  1. Floyd E. Romesberg,
  2. Chengzhi Yu,
  3. Shigeo Matsuda,
  4. Allison A. Henry

Published Online: 1 NOV 2002

DOI: 10.1002/0471142700.nc0105s10

Current Protocols in Nucleic Acid Chemistry

Current Protocols in Nucleic Acid Chemistry

How to Cite

Romesberg, F. E., Yu, C., Matsuda, S. and Henry, A. A. 2002. Development of a Universal Nucleobase and Modified Nucleobases for Expanding the Genetic Code. Current Protocols in Nucleic Acid Chemistry. 10:1.5:1.5.1–1.5.36.

Author Information

  1. The Scripps Research Institute, La Jolla, California

Publication History

  1. Published Online: 1 NOV 2002
  2. Published Print: SEP 2002

Abstract

This unit presents protocols for the synthesis and characterization of nucleosides with unnatural bases in order to develop bases for the expansion of the genetic alphabet or for nonselective pairing opposite natural bases. Protocols describe the design, synthesis, and characterization of unnatural base pairs involving 1-β-d-2-deoxyribosyl-N- and -C-nucleosides. Determination of the thermodynamic and kinetic parameters of unnatural nucleosides is accomplished by incorporation into oligonucleotides and subsequent evaluation as described herein.