UNIT 1.5 Development of a Universal Nucleobase and Modified Nucleobases for Expanding the Genetic Code
Published Online: 1 NOV 2002
Copyright © 2003 by John Wiley and Sons, Inc.
Lab Protocol Title
Current Protocols in Nucleic Acid Chemistry
How to Cite
Romesberg, F. E., Yu, C., Matsuda, S. and Henry, A. A. 2002. Development of a Universal Nucleobase and Modified Nucleobases for Expanding the Genetic Code. Current Protocols in Nucleic Acid Chemistry. 10:1.5:1.5.1–1.5.36.
- Published Online: 1 NOV 2002
- Published Print: SEP 2002
This unit presents protocols for the synthesis and characterization of nucleosides with unnatural bases in order to develop bases for the expansion of the genetic alphabet or for nonselective pairing opposite natural bases. Protocols describe the design, synthesis, and characterization of unnatural base pairs involving 1-β-d-2-deoxyribosyl-N- and -C-nucleosides. Determination of the thermodynamic and kinetic parameters of unnatural nucleosides is accomplished by incorporation into oligonucleotides and subsequent evaluation as described herein.