UNIT 1.17 Nucleobase-Caged Phosphoramidites for Oligonucleotide Synthesis
Published Online: 1 JUN 2007
Copyright © 2007 by John Wiley and Sons, Inc.
Lab Protocol Title
Current Protocols in Nucleic Acid Chemistry
How to Cite
Heckel, A. 2007. Nucleobase-Caged Phosphoramidites for Oligonucleotide Synthesis. Current Protocols in Nucleic Acid Chemistry. 29:1.17:1.17.1–1.17.26.
- Published Online: 1 JUN 2007
- Published Print: JUN 2007
Caged compounds are biologically active agents bearing a photolabile group in a strategic position, which makes them temporarily inactive. These compounds can then be delivered to a biological sample without immediately generating an effect. When the sample is then irradiated, e.g., with a laser in a (confocal) microscope, the activity of the substance is released with exact spatiotemporal and dose control. This unit deals with the synthesis of protected nucleoside phosphoramidites bearing a caging group on the nucleobase, which prevents the nucleobases from forming normal Watson-Crick base pairs. These amidites can be used to generate caged oligodeoxynucleotides with a transitory local perturbation that adds an element of spatiotemporal control to oligonucleotide-based applications.
- temporary mismatch;
- spatiotemporal control;