UNIT 1.22 O6-(Benzotriazol-1-yl)inosine Derivatives for C6 Modification of Purine Nucleosides
Published Online: 1 MAR 2009
Copyright © 2009 by John Wiley & Sons, Inc.
Lab Protocol Title
Current Protocols in Nucleic Acid Chemistry
How to Cite
Bae, S., Chaturvedi, S. and Lakshman, M. K. 2009. O6-(Benzotriazol-1-yl)inosine Derivatives for C6 Modification of Purine Nucleosides. Current Protocols in Nucleic Acid Chemistry. 36:1.22:1.22.1–1.22.23.
- Published Online: 1 MAR 2009
- Published Print: MAR 2009
A new class of reactive nucleosides, O6-(benzotriazol-1-yl) derivatives of inosine and 2′-deoxyinosine, have been developed via reaction of silyl-protected or unprotected inosine and 2′-deoxyinosine with 1H-benzotriazol-1-yloxy-tris(dimethylamino)phosphonium hexafluorophosphate (BOP). Alternatively, the silyl-protected O6-(benzotriazol-1-yl) derivatives can be synthesized via reaction of protected inosine and 2′-deoxyinosine with triphenylphosphine/iodine/1-hydroxybenzotriazole. These new O6-(benzotriazol-1-yl) inosine derivatives are excellent reagents for the synthesis of other nucleoside analogues via SNAr reaction with a range of nucleophiles. Curr. Protoc. Nucleic Acid Chem. 36:1.22.1-1.22.23. © 2009 by John Wiley & Sons, Inc.
- reactive nucleosides;
- convertible nucleosides;