Unit

UNIT 1.25 Synthesis of the Tellurium-Derivatized Phosphoramidites and Their Incorporation into DNA Oligonucleotides

  1. Sibo Jiang,
  2. Jia Sheng,
  3. Zhen Huang

Published Online: 1 DEC 2011

DOI: 10.1002/0471142700.nc0125s47

Current Protocols in Nucleic Acid Chemistry

Current Protocols in Nucleic Acid Chemistry

How to Cite

Jiang, S., Sheng, J. and Huang, Z. 2011. Synthesis of the Tellurium-Derivatized Phosphoramidites and Their Incorporation into DNA Oligonucleotides. Current Protocols in Nucleic Acid Chemistry. 47:1.25:1.25.1–1.25.16.

Author Information

  1. Department of Chemistry, Georgia State University, Atlanta, Georgia

Publication History

  1. Published Online: 1 DEC 2011
  2. Published Print: DEC 2011

Abstract

In this unit, an efficient method for the synthesis of 2′-tellerium-modified phosphoramidite and its incorporation into oligonucleotide are presented. We choose 5′-O-DMTr-2,2′-anhydro-uridine and -thymidine nucleosides (S.1, S.2) as starting materials due to their easy preparation. The 5′-O-DMTr-2,2′-anhydro-uridine and -thymidine can be converted to the corresponding 2′-tellerium-derivatized nucleosides by treating with the telluride nucleophiles. Subsequently, the 2′-Te-nucleosides can be transformed into 3′-phosphoramidites, which are the building blocks for DNA/RNA synthesis. The DNA synthesis, purification, and applications of oligonucleotides containing 2′-Te-U or 2′-Te-T are described in the protocol. Curr. Protoc. Nucleic Acid Chem. 47:1.25.1-1.25.16. © 2011 by John Wiley & Sons, Inc.

Keywords:

  • tellurium nucleic acid;
  • nucleic acid modification;
  • Te-derivatized DNA;
  • nucleic acid X-ray crystallography;
  • DNA damaging;
  • conductive DNA;
  • DNA imaging