Unit

UNIT 2.2 Protection of 2′-Hydroxy Functions of Ribonucleosides

  1. Colin B. Reese

Published Online: 1 MAY 2001

DOI: 10.1002/0471142700.nc0202s00

Current Protocols in Nucleic Acid Chemistry

Current Protocols in Nucleic Acid Chemistry

How to Cite

Reese, C. B. 2001. Protection of 2′-Hydroxy Functions of Ribonucleosides. Current Protocols in Nucleic Acid Chemistry. 00:2.2:2.2.1–2.2.24.

Author Information

  1. King's College London, London, United Kingdom

Publication History

  1. Published Online: 1 MAY 2001
  2. Published Print: FEB 2000

Abstract

The main purpose of this article is to discuss 2′-protection in the context of effective oligoribonucleotide synthesis. Emphasis is placed on the 2′-protecting groups of choice in the synthesis of oligo-and polyribonucleotides, and the requirements that a protective group must satisfy to become the 2′-hydroxyl-protecting group of choice. Finally, the unit discusses the issue of 2′-O-acyl and 2′-O-silyl group migration to the 3′-hydroxy function of ribonucleosides during protection, along with the consequences of the conditions used for their removal on the stability of internucleotide linkages.