Unit

UNIT 2.3 Protection of 5′-Hydroxy Functions of Nucleosides

  1. H. Seliger

Published Online: 1 MAY 2001

DOI: 10.1002/0471142700.nc0203s00

Current Protocols in Nucleic Acid Chemistry

Current Protocols in Nucleic Acid Chemistry

How to Cite

Seliger, H. 2001. Protection of 5′-Hydroxy Functions of Nucleosides. Current Protocols in Nucleic Acid Chemistry. 00:2.3:2.3.1–2.3.34.

Author Information

  1. University of Ulm, Ulm, Germany

Publication History

  1. Published Online: 1 MAY 2001
  2. Published Print: FEB 2000

Abstract

The 5-hydroxy group is the primary hydroxy group of nucleosides. It is mandatory to protect 5-hydroxyls in all methods of oligonucleotide synthesis that require nucleoside synthons. This unit discusses a wide variety of acid-labile and base-labile protecting groups, as well as enzymatic methods for 5-protection and deprotection.