UNIT 2.5 2′-Hydroxyl-Protecting Groups that are Either Photochemically Labile or Sensitive to Fluoride Ions
Published Online: 1 MAY 2001
Copyright © 2003 by John Wiley and Sons, Inc.
Lab Protocol Title
Current Protocols in Nucleic Acid Chemistry
How to Cite
Miller, T. J., Schwartz, M. E. and Gough, G. R. 2001. 2′-Hydroxyl-Protecting Groups that are Either Photochemically Labile or Sensitive to Fluoride Ions. Current Protocols in Nucleic Acid Chemistry. 3:2.5:2.5.1–2.5.36.
- Published Online: 1 MAY 2001
- Published Print: JAN 2001
Protected ribonucleotide monomers are more difficult to obtain than their 2′-deoxy counterparts because of the need to protect the 2′-hydroxy function. This unit describes the stepwise preparation of suitably 2′-protected ribonucleosides using two protecting groups: 2-nitrobenzyloxymethyl (NBOM) and tert-butyldimethylsilyl (TBDMS). In addition, details are given for protecting the 5′-hydroxyl and the nucleobase, yielding nucleosides that are easily converted to phosphoramidite or H-phosphonate derivatives for automated oligoribonucleotide synthesis.