UNIT 2.7 Deoxyribonucleoside Phosphoramidites

  1. Andrzej Wilk1,
  2. Andrzej Grajkowski1,
  3. Marcin K. Chmielewski1,
  4. Serge L. Beaucage1,
  5. Lawrence R. Phillips2

Published Online: 1 MAY 2001

DOI: 10.1002/0471142700.nc0207s04

Current Protocols in Nucleic Acid Chemistry

Current Protocols in Nucleic Acid Chemistry

How to Cite

Wilk, A., Grajkowski, A., Chmielewski, M. K., Beaucage, S. L. and Phillips, L. R. 2001. Deoxyribonucleoside Phosphoramidites. Current Protocols in Nucleic Acid Chemistry. 4:2.7:2.7.1–2.7.12.

Author Information

  1. 1

    Food and Drug Administration, Bethesda, Maryland

  2. 2

    National Cancer Institute, Frederick, Maryland

Publication History

  1. Published Online: 1 MAY 2001
  2. Published Print: APR 2001


The detailed preparation of deoxyribonucleoside phosphoramidites bearing a 4-[N-methyl-N-(2,2,2-trifluoroacetyl)amino]butyl group for P(III) protection is presented. The use of this group circumvents nucleobase alkylation during oligonucleotide deprotection. Two syntheses of phosphoramidites starting from either a phosphordichloridite precursor or a bis-(N,N-diisopropylamino)chlorophosphine intermediate are described for the phosphinylation of suitably protected deoxyribonucleosides.