UNIT 2.7 Deoxyribonucleoside Phosphoramidites
Published Online: 1 MAY 2001
Copyright © 2003 by John Wiley and Sons, Inc.
Lab Protocol Title
Current Protocols in Nucleic Acid Chemistry
How to Cite
Wilk, A., Grajkowski, A., Chmielewski, M. K., Beaucage, S. L. and Phillips, L. R. 2001. Deoxyribonucleoside Phosphoramidites. Current Protocols in Nucleic Acid Chemistry. 4:2.7.1–2.7.12.
- Published Online: 1 MAY 2001
- Published Print: APR 2001
The detailed preparation of deoxyribonucleoside phosphoramidites bearing a 4-[N-methyl-N-(2,2,2-trifluoroacetyl)amino]butyl group for P(III) protection is presented. The use of this group circumvents nucleobase alkylation during oligonucleotide deprotection. Two syntheses of phosphoramidites starting from either a phosphordichloridite precursor or a bis-(N,N-diisopropylamino)chlorophosphine intermediate are described for the phosphinylation of suitably protected deoxyribonucleosides.