Unit

UNIT 2.8 Regioselective 2′-Silylation of Purine Ribonucleosides for Phosphoramidite RNA Synthesis

  1. Barbara L. Gaffney,
  2. Roger A. Jones

Published Online: 1 NOV 2001

DOI: 10.1002/0471142700.nc0208s06

Current Protocols in Nucleic Acid Chemistry

Current Protocols in Nucleic Acid Chemistry

How to Cite

Gaffney, B. L. and Jones, R. A. 2001. Regioselective 2′-Silylation of Purine Ribonucleosides for Phosphoramidite RNA Synthesis. Current Protocols in Nucleic Acid Chemistry. 6:2.8:2.8.1–2.8.13.

Author Information

  1. Rutgers, The State University of New Jersey, Piscataway, New Jersey

Publication History

  1. Published Online: 1 NOV 2001
  2. Published Print: SEP 2001

Abstract

This unit describes high-yield procedures for protection of purine ribonucleosides based on a reaction that allows concomitant highly regioselective 2′-silylation and 3′-phosphitylation to give, in one step, monomers that are ready for H-phosphonate synthesis. For preparation of phosphoramidites, the H-phosphonate monoester is cleaved without silyl migration to give intermediates ready for phosphitylation by standard methods.