UNIT 2.8 Regioselective 2′-Silylation of Purine Ribonucleosides for Phosphoramidite RNA Synthesis
Published Online: 1 NOV 2001
Copyright © 2003 by John Wiley and Sons, Inc.
Lab Protocol Title
Current Protocols in Nucleic Acid Chemistry
How to Cite
Gaffney, B. L. and Jones, R. A. 2001. Regioselective 2′-Silylation of Purine Ribonucleosides for Phosphoramidite RNA Synthesis. Current Protocols in Nucleic Acid Chemistry. 6:2.8:2.8.1–2.8.13.
- Published Online: 1 NOV 2001
- Published Print: SEP 2001
This unit describes high-yield procedures for protection of purine ribonucleosides based on a reaction that allows concomitant highly regioselective 2′-silylation and 3′-phosphitylation to give, in one step, monomers that are ready for H-phosphonate synthesis. For preparation of phosphoramidites, the H-phosphonate monoester is cleaved without silyl migration to give intermediates ready for phosphitylation by standard methods.