UNIT 3.1 Solid-Phase Supports for Oligonucleotide Synthesis

  1. Richard T. Pon

Published Online: 1 MAY 2001

DOI: 10.1002/0471142700.nc0301s00

Current Protocols in Nucleic Acid Chemistry

Current Protocols in Nucleic Acid Chemistry

How to Cite

Pon, R. T. 2001. Solid-Phase Supports for Oligonucleotide Synthesis. Current Protocols in Nucleic Acid Chemistry. 00:3.1:3.1.1–3.1.28.

Author Information

  1. University of Calgary, Calgary, Alberta, Canada

Publication History

  1. Published Online: 1 MAY 2001
  2. Published Print: FEB 2000

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This unit begins with a discussion of the advantages and disadvantages of oligonucleotide synthesis using solid supports. The physical and chemical properties of solid-phase supports are discussed in terms of their suitability for oligonucleotide synthesis. In addition, the unit outlines the properties of linkers used for transient or permanent attachment of properly protected nucleosides to the derivatized support, as well as strategies for coupling nucleosides to linkers and conditions for the release of synthetic oligonucleotides from specific supports.