UNIT 3.2 Attachment of Nucleosides to Solid-Phase Supports

  1. Richard T. Pon

Published Online: 1 MAY 2001

DOI: 10.1002/0471142700.nc0302s00

Current Protocols in Nucleic Acid Chemistry

Current Protocols in Nucleic Acid Chemistry

How to Cite

Pon, R. T. 2001. Attachment of Nucleosides to Solid-Phase Supports. Current Protocols in Nucleic Acid Chemistry. 00:3.2:3.2.1–3.2.23.

Author Information

  1. University of Calgary, Calgary, Alberta, Canada

Publication History

  1. Published Online: 1 MAY 2001
  2. Published Print: FEB 2000

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Specific step-by-step instructions are given for coupling nucleosides to LCAA-CPG supports (supports consisting of a long-chain alkylamine linked to controlled-pore glass). Protocols are given for a succinic acid linker and a hydroquinone-O,O′-diacetic acid linker. The former is the most widely used linker arm, and the starting materials are widely available. The latter offers greater compatibility with base-sensitive sequence modifications and great synthetic throughput because it can be cleaved under milder and faster conditions. Additional guidelines are given for selecting a linker arm and coupling protocol. Almost any application requiring synthetic oligonucletodies can be satisfied using one of these linker arms.