Unit

UNIT 3.7 Synthesis of Oligoribonucleotides Using the 2-Nitrobenzyloxymethyl Group for 2′-Hydroxyl Protection

  1. Tod J. Miller,
  2. Miriam E. Schwartz,
  3. Geoffrey R. Gough

Published Online: 1 MAY 2001

DOI: 10.1002/0471142700.nc0307s03

Current Protocols in Nucleic Acid Chemistry

Current Protocols in Nucleic Acid Chemistry

How to Cite

Miller, T. J., Schwartz, M. E. and Gough, G. R. 2001. Synthesis of Oligoribonucleotides Using the 2-Nitrobenzyloxymethyl Group for 2′-Hydroxyl Protection. Current Protocols in Nucleic Acid Chemistry. 3:3.7:3.7.1–3.7.8.

Author Information

  1. Purdue University, West Lafayette, Indiana

Publication History

  1. Published Online: 1 MAY 2001
  2. Published Print: JAN 2001

Abstract

RNA can be rapidly synthesized using protected ribonucleoside phosphoramidite monomers that carry 2-nitrobenzyloxymethyl groups on their 2-hydroxyls. These protecting groups are removed from the oligoribonucleotide products by exposure to long-wave UV light.