Unit

UNIT 3.10 DNA Synthesis Without Base Protection

  1. Mitsuo Sekine

Published Online: 1 OCT 2004

DOI: 10.1002/0471142700.nc0310s18

Current Protocols in Nucleic Acid Chemistry

Current Protocols in Nucleic Acid Chemistry

How to Cite

Sekine, M. 2004. DNA Synthesis Without Base Protection. Current Protocols in Nucleic Acid Chemistry. 18:3.10:3.10.1–3.10.15.

Author Information

  1. Tokyo Institute of Technology, Yokohama, Japan

Publication History

  1. Published Online: 1 OCT 2004
  2. Published Print: SEP 2004

Abstract

DNA synthesis can be achieved by using O-selective methods for internucleotide bond formation. This greatly simplifies the synthesis of oligodeoxyribonucleotides by eliminating the need for nucleobase protection and deprotection steps. This unit describes strategies that can be used for DNA synthesis without base protection. The discussion includes synthesis of phosphoramidite and H-phosphonate monomers, solid-phase assembly by the phosphoramidite and H-phosphonate methods, and future prospects for DNA synthesis using N-unprotected approaches.

Keywords:

  • O-selectivity;
  • DNA synthesis;
  • base protection;
  • internucleotide bond