UNIT 3.21 Safe Deprotection Strategy for the Tert-Butyldimethylsilyl (TBS) Group During RNA Synthesis
Published Online: 1 JUN 2012
Copyright © 2012 by John Wiley & Sons, Inc.
Lab Protocol Title
Current Protocols in Nucleic Acid Chemistry
How to Cite
Zewge, D. 2012. Safe Deprotection Strategy for the Tert-Butyldimethylsilyl (TBS) Group During RNA Synthesis. Current Protocols in Nucleic Acid Chemistry. 49:3.21:3.21.1–3.21.11.
- Published Online: 1 JUN 2012
This unit describes two protocols for the deprotection of 2′-O-TBS groups in oligoribonucleotides under mild conditions. Desilylation using ammonium fluoride is applicable to fully protected “RNA only” substrates and desilylation using potassium fluoride is applicable to “mixed RNA/non-RNA” substrates. Characterization of products is accomplished using LC/MS, RP HPLC and SAX HPLC. Curr. Protoc. Nucleic Acid Chem. 49:3.21.1-3.21.11. © 2012 by John Wiley & Sons, Inc.
- “RNA only”;
- “mixed RNA/non-RNA” substrates;