UNIT 4.2 Modification of the 5′ Terminus of Oligonucleotides for Attachment of Reporter and Conjugate Groups
Published Online: 1 MAY 2001
Copyright © 2003 by John Wiley and Sons, Inc.
Lab Protocol Title
Current Protocols in Nucleic Acid Chemistry
How to Cite
Thuong, N. T. and Asseline, U. 2001. Modification of the 5′ Terminus of Oligonucleotides for Attachment of Reporter and Conjugate Groups. Current Protocols in Nucleic Acid Chemistry. 00:4.2:4.2.1–4.2.33.
- Published Online: 1 MAY 2001
- Published Print: FEB 2000
Reporter and conjugate groups can be added directly to the 5′ terminus of oligonucleotides by appropriate modification. Conjugate groups can be used to increase the affinity of complementary strands, induce irreversible modification of target sequences, or enable sequences to recognize and permeate target cell membranes. This overview discusses the 5′ modifications that can be used and strategies for the covalent attachment of ligands to the modified oligonucleotides. Step-by-step protocols for attachment of conjugate groups are given elsewhere in the series.