Unit

UNIT 4.2 Modification of the 5′ Terminus of Oligonucleotides for Attachment of Reporter and Conjugate Groups

  1. Nguyen T. Thuong,
  2. Ulysse Asseline

Published Online: 1 MAY 2001

DOI: 10.1002/0471142700.nc0402s00

Current Protocols in Nucleic Acid Chemistry

Current Protocols in Nucleic Acid Chemistry

How to Cite

Thuong, N. T. and Asseline, U. 2001. Modification of the 5′ Terminus of Oligonucleotides for Attachment of Reporter and Conjugate Groups. Current Protocols in Nucleic Acid Chemistry. 00:4.2:4.2.1–4.2.33.

Author Information

  1. Centre de Biophysique Moléculaire, CNRS, Orléans, France

Publication History

  1. Published Online: 1 MAY 2001
  2. Published Print: FEB 2000

Abstract

Reporter and conjugate groups can be added directly to the 5′ terminus of oligonucleotides by appropriate modification. Conjugate groups can be used to increase the affinity of complementary strands, induce irreversible modification of target sequences, or enable sequences to recognize and permeate target cell membranes. This overview discusses the 5′ modifications that can be used and strategies for the covalent attachment of ligands to the modified oligonucleotides. Step-by-step protocols for attachment of conjugate groups are given elsewhere in the series.