Unit

UNIT 4.3 Direct Attachment of Conjugate Groups to the 5′ Terminus of Oligodeoxyribonucleotides

  1. Ulysse Asseline,
  2. Nguyen T. Thuong

Published Online: 1 MAY 2001

DOI: 10.1002/0471142700.nc0403s00

Current Protocols in Nucleic Acid Chemistry

Current Protocols in Nucleic Acid Chemistry

How to Cite

Asseline, U. and Thuong, N. T. 2001. Direct Attachment of Conjugate Groups to the 5′ Terminus of Oligodeoxyribonucleotides. Current Protocols in Nucleic Acid Chemistry. 00:4.3:4.3.1–4.3.16.

Author Information

  1. Centre de Biophysique Moléculaire, CNRS, Orléans, France

Publication History

  1. Published Online: 1 MAY 2001
  2. Published Print: FEB 2000

Abstract

This unit gives protocols for the attachment of intercalating and photoreactive conjugate groups to oligodeoxyribonucleotides. Protocols are given for acridine- and psoralen-conjugated oligonucleotides, and include attachment of the linker, preparation of the phosphoramidite, coupling to the oligonucleotide, deprotection, purification, and characterization.