UNIT 4.3 Direct Attachment of Conjugate Groups to the 5′ Terminus of Oligodeoxyribonucleotides
Published Online: 1 MAY 2001
Copyright © 2003 by John Wiley and Sons, Inc.
Lab Protocol Title
Current Protocols in Nucleic Acid Chemistry
How to Cite
Asseline, U. and Thuong, N. T. 2001. Direct Attachment of Conjugate Groups to the 5′ Terminus of Oligodeoxyribonucleotides. Current Protocols in Nucleic Acid Chemistry. 00:4.3:4.3.1–4.3.16.
- Published Online: 1 MAY 2001
- Published Print: FEB 2000
This unit gives protocols for the attachment of intercalating and photoreactive conjugate groups to oligodeoxyribonucleotides. Protocols are given for acridine- and psoralen-conjugated oligonucleotides, and include attachment of the linker, preparation of the phosphoramidite, coupling to the oligonucleotide, deprotection, purification, and characterization.