Unit

UNIT 4.4 Synthesis and Characterization of Chimeric 2-5A-DNA Oligonucleotides

  1. Mark R. Player,
  2. Paul F. Torrence

Published Online: 1 MAY 2001

DOI: 10.1002/0471142700.nc0404s01

Current Protocols in Nucleic Acid Chemistry

Current Protocols in Nucleic Acid Chemistry

How to Cite

Player, M. R. and Torrence, P. F. 2001. Synthesis and Characterization of Chimeric 2-5A-DNA Oligonucleotides. Current Protocols in Nucleic Acid Chemistry. 1:4.4:4.4.1–4.4.23.

Author Information

  1. Northern Arizona University, Flagstaff, Arizona

Publication History

  1. Published Online: 1 MAY 2001
  2. Published Print: JUL 2000

Abstract

This unit provides protocols for the synthesis and characterization of 2-5A-antisense nucleic acids. These chimeric oligonucleotides consist of 2′,5′-phosphodiester-linked oligoadenylates ligated to 3′,5′-deoxyribonucleotides and are readily prepared using phosphoramidite chemistry on CPG solid supports. The 3′,5′-deoxyribonucleotide functions as the antisense domain to target a given mRNA sequence, while the 2′,5′-phosphodiester-linked oligoadenylate serves to locally activate 2-5A-dependent RNase L, causing the targeted sequence to be cleaved.