UNIT 4.4 Synthesis and Characterization of Chimeric 2-5A-DNA Oligonucleotides
Published Online: 1 MAY 2001
Copyright © 2003 by John Wiley and Sons, Inc.
Lab Protocol Title
Current Protocols in Nucleic Acid Chemistry
How to Cite
Player, M. R. and Torrence, P. F. 2001. Synthesis and Characterization of Chimeric 2-5A-DNA Oligonucleotides. Current Protocols in Nucleic Acid Chemistry. 1:4.4:4.4.1–4.4.23.
- Published Online: 1 MAY 2001
- Published Print: JUL 2000
This unit provides protocols for the synthesis and characterization of 2-5A-antisense nucleic acids. These chimeric oligonucleotides consist of 2′,5′-phosphodiester-linked oligoadenylates ligated to 3′,5′-deoxyribonucleotides and are readily prepared using phosphoramidite chemistry on CPG solid supports. The 3′,5′-deoxyribonucleotide functions as the antisense domain to target a given mRNA sequence, while the 2′,5′-phosphodiester-linked oligoadenylate serves to locally activate 2-5A-dependent RNase L, causing the targeted sequence to be cleaved.