Unit

UNIT 4.7 Synthesis and Purification of Oligonucleotide N3′[RIGHTWARDS ARROW]P5′ Phosphoramidates and their Phosphodiester and Phosphorothioate Chimeras

  1. Karen L. Fearon,
  2. Jeffrey S. Nelson

Published Online: 1 MAY 2001

DOI: 10.1002/0471142700.nc0407s03

Current Protocols in Nucleic Acid Chemistry

Current Protocols in Nucleic Acid Chemistry

How to Cite

Fearon, K. L. and Nelson, J. S. 2001. Synthesis and Purification of Oligonucleotide N3′[RIGHTWARDS ARROW]P5′ Phosphoramidates and their Phosphodiester and Phosphorothioate Chimeras. Current Protocols in Nucleic Acid Chemistry. 3:4.7:4.7.1–4.7.40.

Author Information

  1. Lynx Therapeutics, Hayward, California

Publication History

  1. Published Online: 1 MAY 2001
  2. Published Print: JAN 2001

Abstract

This unit describes the synthesis and purification of oligonucleotide N3′[RIGHTWARDS ARROW]P5′ phosphoramidates, wherein each 3′-oxygen is replaced by a 3′-amine in the 2′-deoxyribose ring. The synthesis of required monomers and application of the method to preparation of phosphodiester- and phosphorothioate-containing chimera of phosphoramidate is also reported.