Unit

UNIT 4.8 Incorporation of Halogenoalkyl, 2-Pyridyldithioalkyl, or Isothiocyanate Linkers into Ligands

  1. Ulysee Asseline,
  2. Nguyen T. Thuong

Published Online: 1 AUG 2001

DOI: 10.1002/0471142700.nc0408s05

Current Protocols in Nucleic Acid Chemistry

Current Protocols in Nucleic Acid Chemistry

How to Cite

Asseline, U. and Thuong, N. T. 2001. Incorporation of Halogenoalkyl, 2-Pyridyldithioalkyl, or Isothiocyanate Linkers into Ligands. Current Protocols in Nucleic Acid Chemistry. 5:4.8:4.8.1–4.8.15.

Author Information

  1. Centre de Biophysique Moléculaire, CNRS, Orléans, France

Publication History

  1. Published Online: 1 AUG 2001
  2. Published Print: JUN 2001

Abstract

Ligands can be introduced at the 5' terminus of an oligonucleotide by adding a linker to the ligand and modifying the 5' terminus of the oligonucleotide. These are then reacted to give the ligand-oligonucleotide conjugate. The addition of appropriate linkers to ligands is described in this unit. 5'Modification of the oligonucleotide and the final reaction that produces the ligand-conjugated oligonucleotide are described elsewhere in the series. This approach is particularly useful when there is a limited amount of ligand available, when the ligand is sensitive to chemical conditions required for oligonucleotide deprotection, or when the ligand is weakly soluble in solvents required for phosphoramidite- or H-phosphonate-mediated oligonucleotide synthesis.