Unit

UNIT 4.10 Conjugation of 5′-Functionalized Oligodeoxyribonucleotides with Properly Functionalized Ligands

  1. Ulysse Asseline,
  2. Nguyen T. Thuong

Published Online: 1 FEB 2002

DOI: 10.1002/0471142700.nc0410s07

Current Protocols in Nucleic Acid Chemistry

Current Protocols in Nucleic Acid Chemistry

How to Cite

Asseline, U. and Thuong, N. T. 2002. Conjugation of 5′-Functionalized Oligodeoxyribonucleotides with Properly Functionalized Ligands. Current Protocols in Nucleic Acid Chemistry. 7:4.10:4.10.1–4.10.23.

Author Information

  1. Centre de Biophysique Moleculaire, CNRS Orléans, France

Publication History

  1. Published Online: 1 FEB 2002
  2. Published Print: DEC 2001

Abstract

This unit reports the synthesis of oligodeoxyribonucleotides covalently linked via their 5′ termini to various ligands such as intercalating agents, reactive groups, or labels. Methods for incorporation of halogenoalkyl, isothiocyanate, and 2-pyridyldisulfide linkers onto ligands, and addition of amino, carboxyl, thiophosphate, phosphate, and masked thiol groups at the 5 terminus of an oligodeoxyribonucleotide are described elsewhere in the series. This unit reports procedures for coupling the ligands and oligonucleotides, as well as details for purification and characterization of the final products.