UNIT 4.12 Locked Nucleic Acids: Synthesis and Characterization of LNA-T Diol
Published Online: 1 MAY 2002
Copyright © 2003 by John Wiley and Sons, Inc.
Lab Protocol Title
Current Protocols in Nucleic Acid Chemistry
How to Cite
Pfundheller, H. M. and Lomholt, C. 2002. Locked Nucleic Acids: Synthesis and Characterization of LNA-T Diol. Current Protocols in Nucleic Acid Chemistry. 8:4.12:4.12.1–4.12.16.
- Published Online: 1 MAY 2002
- Published Print: MAR 2002
Locked nucleic acids (LNAs) are RNA derivatives that have an O-methylene linkage between the 2 and 4 positions of the ribose. This leads to exceptionally high-affinity binding to complementary sequences. LNAs are synthesized from a commercially available sugar, 1,2:5,6-di-O-isopropylidene-a-d-allofuranose. An efficient and simplified procedure is presented for synthesizing a glycol donor that can be used for synthesis of a variety of LNA monomers. Then, as an example, the synthesis of the thymidine analog of LNA from this glycol donor is presented. The protocols give high yields of the desired products and avoid the use of time-consuming column chromatography.