Unit

UNIT 4.12 Locked Nucleic Acids: Synthesis and Characterization of LNA-T Diol

  1. Henrik M. Pfundheller,
  2. Christian Lomholt

Published Online: 1 MAY 2002

DOI: 10.1002/0471142700.nc0412s08

Current Protocols in Nucleic Acid Chemistry

Current Protocols in Nucleic Acid Chemistry

How to Cite

Pfundheller, H. M. and Lomholt, C. 2002. Locked Nucleic Acids: Synthesis and Characterization of LNA-T Diol. Current Protocols in Nucleic Acid Chemistry. 8:4.12:4.12.1–4.12.16.

Author Information

  1. Exiqon A/S, Vedbaek, Denmark

Publication History

  1. Published Online: 1 MAY 2002
  2. Published Print: MAR 2002

Abstract

Locked nucleic acids (LNAs) are RNA derivatives that have an O-methylene linkage between the 2 and 4 positions of the ribose. This leads to exceptionally high-affinity binding to complementary sequences. LNAs are synthesized from a commercially available sugar, 1,2:5,6-di-O-isopropylidene-a-d-allofuranose. An efficient and simplified procedure is presented for synthesizing a glycol donor that can be used for synthesis of a variety of LNA monomers. Then, as an example, the synthesis of the thymidine analog of LNA from this glycol donor is presented. The protocols give high yields of the desired products and avoid the use of time-consuming column chromatography.