UNIT 4.13 Cellular Delivery of Locked Nucleic Acids (LNAs)
Published Online: 1 AUG 2002
Copyright © 2003 by John Wiley and Sons, Inc.
Lab Protocol Title
Current Protocols in Nucleic Acid Chemistry
How to Cite
Braasch, D. A. and Corey, D. R. 2002. Cellular Delivery of Locked Nucleic Acids (LNAs). Current Protocols in Nucleic Acid Chemistry. 9:4.13:4.13.1–4.13.9.
- Published Online: 1 AUG 2002
- Published Print: JUN 2002
Locked nucleic acids (LNAs) are RNA derivatives that have an O-methylene linkage between the 2 and 4 positions of the ribose. This leads to exceptionally high-affinity binding to complementary sequences. They are synthesized using standard DNA/RNA synthesis methods, and have a negatively charged backbone that confers good solubility. This unit describes a method for the introduction of LNA oligomers into cells. A support protocol also describes the determination of melting temperatures for LNA oligomers.