Unit

UNIT 4.15 Solid-Phase Synthesis of 2′-Deoxy-2′-fluoro- β-D-Oligoarabinonucleotides (2′F-ANA) and Their Phosphorothioate Derivatives

  1. Ekaterina Viazovkina,
  2. Maria M. Mangos,
  3. Mohamed I. Elzagheid,
  4. Masad J. Damha

Published Online: 1 NOV 2002

DOI: 10.1002/0471142700.nc0415s10

Current Protocols in Nucleic Acid Chemistry

Current Protocols in Nucleic Acid Chemistry

How to Cite

Viazovkina, E., Mangos, M. M., Elzagheid, M. I. and Damha, M. J. 2002. Solid-Phase Synthesis of 2′-Deoxy-2′-fluoro- β-D-Oligoarabinonucleotides (2′F-ANA) and Their Phosphorothioate Derivatives. Current Protocols in Nucleic Acid Chemistry. 10:4.15:4.15.1–4.15.22.

Author Information

  1. McGill University, Montreal, Canada

Publication History

  1. Published Online: 1 NOV 2002
  2. Published Print: SEP 2002

Abstract

This unit describes the chemical synthesis of 2'-deoxy-2'-fluoro-b-D-oligoarabinonucleotides (2'F-ANA), both with phosphodiester and phosphorothioate linkages. The protocols described herein include araF phosphoramidite preparation, assembly on DNA synthesizers, and final deprotection and purification of oligonucleotides.