Unit

UNIT 4.18 Synthesis of Oligonucleotide Conjugates via Aqueous Diels-Alder Cycloaddition

  1. Michael Leuck,
  2. Andreas Wolter

Published Online: 1 NOV 2003

DOI: 10.1002/0471142700.nc0418s14

Current Protocols in Nucleic Acid Chemistry

Current Protocols in Nucleic Acid Chemistry

How to Cite

Leuck, M. and Wolter, A. 2003. Synthesis of Oligonucleotide Conjugates via Aqueous Diels-Alder Cycloaddition. Current Protocols in Nucleic Acid Chemistry. 14:4.18:4.18.1–4.18.14.

Author Information

  1. Proligo LLC, Boulder, Colorado

Publication History

  1. Published Online: 1 NOV 2003
  2. Published Print: SEP 2003

Abstract

The conjugation of maleimide reporter groups to 5′-diene-modified oligonucleotides via aqueous Diels-Alder cycloaddition is described. Detailed basic protocols are provided for the preparation of a diene-amidite (5′-diene modifier), for the attachment of a diene group to the 5′-terminus of oligonucleotides, and for the conjugation of maleimide reporter groups to diene-modified oligonucleotides.

Keywords:

  • diene-amidite;
  • 5′-diene modifier;
  • Diels-Alder conjugation;
  • 5′-labeling of oligonucleotides