Unit

UNIT 4.29 Methoxyoxalamido Chemistry in the Synthesis of Tethered Phosphoramidites and Functionalized Oligonucleotides

  1. Alan M. Morocho,
  2. Nikolai N. Polushin

Published Online: 1 JUL 2006

DOI: 10.1002/0471142700.nc0429s25

Current Protocols in Nucleic Acid Chemistry

Current Protocols in Nucleic Acid Chemistry

How to Cite

Morocho, A. M. and Polushin, N. N. 2006. Methoxyoxalamido Chemistry in the Synthesis of Tethered Phosphoramidites and Functionalized Oligonucleotides. Current Protocols in Nucleic Acid Chemistry. 25:4.29:4.29.1–4.29.19.

Author Information

  1. Fidelity Systems, Inc., Gaithersburg, Maryland

Publication History

  1. Published Online: 1 JUL 2006
  2. Published Print: JUN 2006

Abstract

A general approach to phosphoramidites tethered with single and multiple linkers through the use of methoxyoxalamido (MOX) chemistry is described. The approach utilizes readily available and inexpensive primary aliphatic amino alcohols and diamines to produce a rich and diverse variety of tethered phosphoramidites. Furthermore, the use of MOX chemistry in a modular fashion enables fairly rapid assembly of compound tethers. All novel phosphoramidites described have been successfully used in automated syntheses of 5′-modified oligonucleotides.

Keywords:

  • methoxyoxalamido (MOX) chemistry;
  • tethered phosphoramidites;
  • 5′-modified oligonucleotides