UNIT 4.29 Methoxyoxalamido Chemistry in the Synthesis of Tethered Phosphoramidites and Functionalized Oligonucleotides
Published Online: 1 JUL 2006
Copyright © 2006 by John Wiley & Sons, Inc.
Lab Protocol Title
Current Protocols in Nucleic Acid Chemistry
How to Cite
Morocho, A. M. and Polushin, N. N. 2006. Methoxyoxalamido Chemistry in the Synthesis of Tethered Phosphoramidites and Functionalized Oligonucleotides. Current Protocols in Nucleic Acid Chemistry. 25:4.29:4.29.1–4.29.19.
- Published Online: 1 JUL 2006
- Published Print: JUN 2006
A general approach to phosphoramidites tethered with single and multiple linkers through the use of methoxyoxalamido (MOX) chemistry is described. The approach utilizes readily available and inexpensive primary aliphatic amino alcohols and diamines to produce a rich and diverse variety of tethered phosphoramidites. Furthermore, the use of MOX chemistry in a modular fashion enables fairly rapid assembly of compound tethers. All novel phosphoramidites described have been successfully used in automated syntheses of 5′-modified oligonucleotides.
- methoxyoxalamido (MOX) chemistry;
- tethered phosphoramidites;
- 5′-modified oligonucleotides