UNIT 4.33 Synthesis of Alkyne- and Azide-Modified Oligonucleotides and Their Cyclization by the CuAAC (Click) Reaction
Published Online: 1 DEC 2008
Copyright © 2008 by John Wiley & Sons, Inc.
Lab Protocol Title
Current Protocols in Nucleic Acid Chemistry
How to Cite
El-Sagheer, A. H. and Brown, T. 2008. Synthesis of Alkyne- and Azide-Modified Oligonucleotides and Their Cyclization by the CuAAC (Click) Reaction. Current Protocols in Nucleic Acid Chemistry. 35:4.33:4.33.1–4.33.21.
- Published Online: 1 DEC 2008
- Published Print: DEC 2008
The Cu(I)-catalyzed alkyne-azide cycloaddition (CuAAC) reaction has been used to synthesize cyclic mini-DNA duplexes. The reaction is carried out on 5′-alkyne-3′-azide-labeled hairpin loop oligonucleotides and proceeds in high yield under mild conditions in as little as 5 min. The resultant duplexes have very high thermal stability and their CD spectra are characteristic of normal B-DNA. Curr. Protoc. Nucleic Acid Chem. 35:4.33.1-4.33.21. © 2008 by John Wiley & Sons, Inc.
- Click chemistry;
- CuAAC reaction;
- oligonucleotide cyclization;