UNIT 4.38 Carbohydrate-Oligonucleotide Conjugates
Published Online: 1 DEC 2009
Copyright © 2009 by John Wiley & Sons, Inc.
Lab Protocol Title
Current Protocols in Nucleic Acid Chemistry
How to Cite
Pourceau, G., Meyer, A., Vasseur, J.-J. and Morvan, F. 2009. Carbohydrate-Oligonucleotide Conjugates. Current Protocols in Nucleic Acid Chemistry. 39:4.38:4.38.1–4.38.25.
- Published Online: 1 DEC 2009
- Published Print: DEC 2009
This unit describes a strategy for attaching two mannose and two galactose residues to an oligonucleotide. This conjugation can be performed at the 5′-end of the oligonucleotide sequence, using modified phosphoramidites. First, the oligonucleotide scaffold is synthesized on solid support using a DNA synthesizer, with commercially available and modified phosphoramidites. After the first “click” reaction with a galactosylated azide derivative on solid support, the bromine atoms are replaced with azides and a second click reaction is performed with propargylated mannose either on solid support or in solution. Additionally, using a monoalkynated solid support, the conjugation with carbohydrate residues can be performed at the 3′-end of the oligonucleotide according to a similar protocol. Curr. Protoc. Nucleic Acid Chem. 39:4.38.1-4.38.25. © 2009 by John Wiley & Sons, Inc.
- click chemistry;