Unit

UNIT 4.38 Carbohydrate-Oligonucleotide Conjugates

  1. Gwladys Pourceau,
  2. Albert Meyer,
  3. Jean-Jacques Vasseur,
  4. François Morvan

Published Online: 1 DEC 2009

DOI: 10.1002/0471142700.nc0438s39

Current Protocols in Nucleic Acid Chemistry

Current Protocols in Nucleic Acid Chemistry

How to Cite

Pourceau, G., Meyer, A., Vasseur, J.-J. and Morvan, F. 2009. Carbohydrate-Oligonucleotide Conjugates. Current Protocols in Nucleic Acid Chemistry. 39:4.38:4.38.1–4.38.25.

Author Information

  1. Institut des Biomolécules Max Mousseron, CNRS, Université Montpellier 1, Université Montpellier 2, Montpellier, France

Publication History

  1. Published Online: 1 DEC 2009
  2. Published Print: DEC 2009

Abstract

This unit describes a strategy for attaching two mannose and two galactose residues to an oligonucleotide. This conjugation can be performed at the 5′-end of the oligonucleotide sequence, using modified phosphoramidites. First, the oligonucleotide scaffold is synthesized on solid support using a DNA synthesizer, with commercially available and modified phosphoramidites. After the first “click” reaction with a galactosylated azide derivative on solid support, the bromine atoms are replaced with azides and a second click reaction is performed with propargylated mannose either on solid support or in solution. Additionally, using a monoalkynated solid support, the conjugation with carbohydrate residues can be performed at the 3′-end of the oligonucleotide according to a similar protocol. Curr. Protoc. Nucleic Acid Chem. 39:4.38.1-4.38.25. © 2009 by John Wiley & Sons, Inc.

Keywords:

  • carbohydrate;
  • oligonucleotide;
  • click chemistry;
  • conjugate;
  • galactose;
  • mannose