Unit

UNIT 4.41 Synthesis of Peptide-Oligonucleotide Conjugates Using a Heterobifunctional Crosslinker

  1. Berea A.R. Williams1,2,
  2. John C. Chaput1,2

Published Online: 1 SEP 2010

DOI: 10.1002/0471142700.nc0441s42

Current Protocols in Nucleic Acid Chemistry

Current Protocols in Nucleic Acid Chemistry

How to Cite

Williams, B. A. and Chaput, J. C. 2010. Synthesis of Peptide-Oligonucleotide Conjugates Using a Heterobifunctional Crosslinker. Current Protocols in Nucleic Acid Chemistry. 42:4.41:4.41.1–4.41.20.

Author Information

  1. 1

    Department of Chemistry and Biochemistry, Arizona State University, Tempe, Arizona

  2. 2

    The Biodesign Institute at Arizona State University, Tempe, Arizona

Publication History

  1. Published Online: 1 SEP 2010
  2. Published Print: SEP 2010

Abstract

Peptide-oligonucleotide conjugates (POCs) are molecular chimeras composed of a nucleic acid moiety covalently attached to a polypeptide moiety. POCs have been used in numerous applications from therapeutics to nanotechnology, and most recently as combinatorial agents in the assembly of bivalent protein affinity reagents. This unit describes the synthesis and purification of POC molecules using the heterobifunctional crosslinking reagent succinimidyl-4-(N-maleimidomethyl)cyclohexane-1-carboxylate (SMCC), which enables amine-modified oligonucleotides to become covalently linked to cysteine-modified polypeptides. This solution-based protocol consists of a two-step synthesis followed by a single purification step. Curr. Protoc. Nucleic Acid Chem. 42:4.41.1-4.41.20. © 2010 by John Wiley & Sons, Inc.

Keywords:

  • peptide-oligonucleotide conjugate;
  • succinimidyl-4-(N-maleimidomethyl)cyclohexane-1-carboxylate (SMCC);
  • fragment conjugation;
  • antisense;
  • DNA nanotechnology;
  • synbodies;
  • nanodisplay