Unit

UNIT 4.43 Preparation of C5-Functionalized Locked Nucleic Acids (LNAs)

  1. Pawan Kumar,
  2. Michael E. Østergaard,
  3. Patrick J. Hrdlicka

Published Online: 1 MAR 2011

DOI: 10.1002/0471142700.nc0443s44

Current Protocols in Nucleic Acid Chemistry

Current Protocols in Nucleic Acid Chemistry

How to Cite

Kumar, P., Østergaard, M. E. and Hrdlicka, P. J. 2011. Preparation of C5-Functionalized Locked Nucleic Acids (LNAs). Current Protocols in Nucleic Acid Chemistry. 44:4.43:4.43.1–4.43.22.

Author Information

  1. University of Idaho, Moscow, Idaho

Publication History

  1. Published Online: 1 MAR 2011
  2. Published Print: MAR 2011

Abstract

Relative to conventional locked nucleic acids (LNAs), C5-functionalized LNAs with small entities at the C5-position display markedly higher (1) duplex thermostability with complementary single-stranded DNA/RNA targets, (2) target specificity, and (3) 3′-exonuclease stability. C5-Functionalized LNAs carrying a polarity-sensitive fluorophore enable sensitive and efficient discrimination of single nucleotide polymorphisms (SNPs) under non-stringent conditions. This unit describes protocols for chemical synthesis of an LNA uridine diol and corresponding C5-functionalized LNA uridine phosphoramidites. A procedure for incorporation of C5-functionalized LNA uridine phosphoramidites into oligodeoxyribonucleotides by automated DNA synthesis is also described. Curr. Protoc. Nucleic Acid Chem. 44:4.43.1-4.43.22. © 2011 by John Wiley & Sons, Inc.

Keywords:

  • antisense;
  • bridged nucleic acid (BNA);
  • conformationally restricted nucleotide;
  • locked nucleic acid (LNA);
  • nucleobase-modified nucleotide;
  • pyrene