UNIT 4.43 Preparation of C5-Functionalized Locked Nucleic Acids (LNAs)
Published Online: 1 MAR 2011
Copyright © 2011 by John Wiley & Sons, Inc.
Lab Protocol Title
Current Protocols in Nucleic Acid Chemistry
How to Cite
Kumar, P., Østergaard, M. E. and Hrdlicka, P. J. 2011. Preparation of C5-Functionalized Locked Nucleic Acids (LNAs). Current Protocols in Nucleic Acid Chemistry. 44:4.43:4.43.1–4.43.22.
- Published Online: 1 MAR 2011
- Published Print: MAR 2011
Relative to conventional locked nucleic acids (LNAs), C5-functionalized LNAs with small entities at the C5-position display markedly higher (1) duplex thermostability with complementary single-stranded DNA/RNA targets, (2) target specificity, and (3) 3′-exonuclease stability. C5-Functionalized LNAs carrying a polarity-sensitive fluorophore enable sensitive and efficient discrimination of single nucleotide polymorphisms (SNPs) under non-stringent conditions. This unit describes protocols for chemical synthesis of an LNA uridine diol and corresponding C5-functionalized LNA uridine phosphoramidites. A procedure for incorporation of C5-functionalized LNA uridine phosphoramidites into oligodeoxyribonucleotides by automated DNA synthesis is also described. Curr. Protoc. Nucleic Acid Chem. 44:4.43.1-4.43.22. © 2011 by John Wiley & Sons, Inc.
- bridged nucleic acid (BNA);
- conformationally restricted nucleotide;
- locked nucleic acid (LNA);
- nucleobase-modified nucleotide;