UNIT 5.3 Engineering Specific Cross-Links in Nucleic Acids Using Glycol Linkers
Published Online: 1 MAY 2001
Copyright © 2003 by John Wiley and Sons, Inc.
Lab Protocol Title
Current Protocols in Nucleic Acid Chemistry
How to Cite
O'Dea, T. and McLaughlin, L. W. 2001. Engineering Specific Cross-Links in Nucleic Acids Using Glycol Linkers. Current Protocols in Nucleic Acid Chemistry. 00:5.3:5.3.1–5.3.8.
- Published Online: 1 MAY 2001
- Published Print: FEB 2000
One of the most convenient methods for generating oligonucleotides possessing intra- or interstrand cross-links is through incorporation of oligoethylene glycol bridges by solid-phase synthesis. The reagents are commercially available or can be synthesized in a few easy synthetic steps. Unlike many other DNA and RNA cross-links, aspects of the structural and thermodynamic impact of modifying nucleic acids with oligoethylene glycols have been studied. This unit covers protection, phosphitylation, and preparation of the glycol linker for oligonucleotide synthesis.