Unit

UNIT 5.7 Engineering Terminal Disulfide Bonds Into DNA

  1. Gary D. Glick

Published Online: 1 AUG 2003

DOI: 10.1002/0471142700.nc0507s13

Current Protocols in Nucleic Acid Chemistry

Current Protocols in Nucleic Acid Chemistry

How to Cite

Glick, G. D. 2003. Engineering Terminal Disulfide Bonds Into DNA. Current Protocols in Nucleic Acid Chemistry. 13:5.7:5.7.1–5.7.13.

Author Information

  1. University of Michigan, Ann Arbor, Michigan

Publication History

  1. Published Online: 1 AUG 2003
  2. Published Print: JUN 2003

Abstract

This protocol presents a simple and general means of modifying nucleic acids with disulfide cross-links. These cross-links serve as powerful tools to probe the structure, dynamics, thermodynamics, folding, and function of DNA and RNA, much in the way that cystine cross-links have been used to study proteins. The chemistry described has been used to synthesize disulfide-cross-linked hairpins and duplexes, higher-order structures such as triplexes, non-ground-state conformations, and tRNAs. since the cross-links form quantitatively by mild air oxidation and do not purturb either secondary or tertiary structure, this modification should prove quite useful for the study of nucleic acids.

Keywords:

  • nucleic acid;
  • disulfide cross-link;
  • structure;
  • dynamics;
  • stability