UNIT 5.7 Engineering Terminal Disulfide Bonds Into DNA
Published Online: 1 AUG 2003
Copyright © 2003 by John Wiley and Sons, Inc.
Lab Protocol Title
Current Protocols in Nucleic Acid Chemistry
How to Cite
Glick, G. D. 2003. Engineering Terminal Disulfide Bonds Into DNA. Current Protocols in Nucleic Acid Chemistry. 13:5.7:5.7.1–5.7.13.
- Published Online: 1 AUG 2003
- Published Print: JUN 2003
This protocol presents a simple and general means of modifying nucleic acids with disulfide cross-links. These cross-links serve as powerful tools to probe the structure, dynamics, thermodynamics, folding, and function of DNA and RNA, much in the way that cystine cross-links have been used to study proteins. The chemistry described has been used to synthesize disulfide-cross-linked hairpins and duplexes, higher-order structures such as triplexes, non-ground-state conformations, and tRNAs. since the cross-links form quantitatively by mild air oxidation and do not purturb either secondary or tertiary structure, this modification should prove quite useful for the study of nucleic acids.
- nucleic acid;
- disulfide cross-link;