UNIT 5.12 DNA Interstrand Cross-Link Formation Using Furan as a Masked Reactive Aldehyde
Published Online: 8 OCT 2013
Copyright © 2013 John Wiley & Sons, Inc. All rights reserved.
Lab Protocol Title
Current Protocols in Nucleic Acid Chemistry
How to Cite
Carrette, L. L., Gyssels, E. and Madder, A. 2013. DNA Interstrand Cross-Link Formation Using Furan as a Masked Reactive Aldehyde. Current Protocols in Nucleic Acid Chemistry. 54:5.12:5.12.1–5.12.16.
- Published Online: 8 OCT 2013
This unit describes a method for interstrand cross-linking between a furan-modified oligonucleotide and its unmodified complement. The synthesis of two furan-modified phosphoramidites, selected based on high cross-linking yield versus improved cross-linking selectivity, is described. The methods allow gram-scale synthesis starting from stable and readily available furan derivatives. Cross-linking requires selective oxidation of the furan moiety to an aldehyde. The masked nature of the latter avoids undesired and off-target reactions, resulting in clean and high-yield cross-link formation. Curr. Protoc. Nucleic Acid Chem. 54:5.12.1-5.12.16. © 2013 by John Wiley & Sons, Inc.