UNIT 6.1 Probing RNA Structure with Chemical Reagents and Enzymes
Published Online: 1 MAY 2001
Copyright © 2003 by John Wiley and Sons, Inc.
Lab Protocol Title
Current Protocols in Nucleic Acid Chemistry
How to Cite
Ziehler, W. A. and Engelke, D. R. 2001. Probing RNA Structure with Chemical Reagents and Enzymes. Current Protocols in Nucleic Acid Chemistry. 00:6.1:6.1.1–6.1.21.
- Published Online: 1 MAY 2001
- Published Print: FEB 2000
This unit provides thorough coverage of the most useful chemical and enzyme probes that can be used to examine RNA secondary and tertiary structure. Footprinting methods are presented using dimethyl sulfate, diethyl pyrocarbonate, ethylnitrosourea, kethoxal, CMCT, and nucleases. For chemical probes, both strand scission and primer extension detection protocols are included.