Unit

UNIT 6.3 Probing RNA Structure by Lead Cleavage

  1. Tao Pan

Published Online: 1 MAY 2001

DOI: 10.1002/0471142700.nc0603s00

Current Protocols in Nucleic Acid Chemistry

Current Protocols in Nucleic Acid Chemistry

How to Cite

Pan, T. 2001. Probing RNA Structure by Lead Cleavage. Current Protocols in Nucleic Acid Chemistry. 00:6.3:6.3.1–6.3.9.

Author Information

  1. University of Chicago, Chicago, Illinois

Publication History

  1. Published Online: 1 MAY 2001
  2. Published Print: FEB 2000

Abstract

Lead cleavage causes a transesterification reaction that breaks the 5′,3′-phosphodiester backbone of RNA, leaving a 2′,3′-cyclic phosphate and a 5′-hydroxyl. Since the efficiency of the reaction at the 2′-hydroxyl is related to steric and chemical constraints on particular 2′-hydroxyls embedded in the RNA, this reaction can be used to examine the structure of individual nucleotides within an RNA molecule. It is a sensitive probe of tertiary RNA structure, provided that Pb2+-binding sites are created in the tertiary structure.