UNIT 6.3 Probing RNA Structure by Lead Cleavage
Published Online: 1 MAY 2001
Copyright © 2003 by John Wiley and Sons, Inc.
Lab Protocol Title
Current Protocols in Nucleic Acid Chemistry
How to Cite
Pan, T. 2001. Probing RNA Structure by Lead Cleavage. Current Protocols in Nucleic Acid Chemistry. 00:6.3:6.3.1–6.3.9.
- Published Online: 1 MAY 2001
- Published Print: FEB 2000
Lead cleavage causes a transesterification reaction that breaks the 5′,3′-phosphodiester backbone of RNA, leaving a 2′,3′-cyclic phosphate and a 5′-hydroxyl. Since the efficiency of the reaction at the 2′-hydroxyl is related to steric and chemical constraints on particular 2′-hydroxyls embedded in the RNA, this reaction can be used to examine the structure of individual nucleotides within an RNA molecule. It is a sensitive probe of tertiary RNA structure, provided that Pb2+-binding sites are created in the tertiary structure.