Unit

UNIT 6.4 Probing Nucleic Acid Structure with Nickel- and Cobalt-Based Reagents

  1. Steven E. Rokita1,
  2. Cynthia J. Burrows2

Published Online: 1 MAY 2001

DOI: 10.1002/0471142700.nc0604s00

Current Protocols in Nucleic Acid Chemistry

Current Protocols in Nucleic Acid Chemistry

How to Cite

Rokita, S. E. and Burrows, C. J. 2001. Probing Nucleic Acid Structure with Nickel- and Cobalt-Based Reagents. Current Protocols in Nucleic Acid Chemistry. 00:6.4:6.4.1–6.4.7.

Author Information

  1. 1

    University of Maryland, College Park, Maryland

  2. 2

    University of Utah, Salt Lake City, Utah

Publication History

  1. Published Online: 1 MAY 2001
  2. Published Print: FEB 2000

Abstract

The use of nickel and cobalt reagents is presented for characterizing the solvent exposure of guanine residues in DNA and RNA. These reagents promote guanine oxidation in the presence of a peracid such as monopersulfate, and the extent of reaction indicates the steric and electronic environment surrounding the N7 and aromatic face of this residue. Since oxidation does not itself perturb target structure or induce strand scission, it is coupled with fragmentation by treatment with piperidine (for smaller polynucleotides) or termination of primer extension (for larger polynucleotides).