UNIT 6.11 Wavelength-Selective Uncaging of Oligonucleotides

  1. Alexandre Rodrigues-Correia,
  2. Patrick Seyfried,
  3. Alexander Heckel

Published Online: 24 JUN 2014

DOI: 10.1002/0471142700.nc0611s57

Current Protocols in Nucleic Acid Chemistry

Current Protocols in Nucleic Acid Chemistry

How to Cite

Rodrigues-Correia, A., Seyfried, P. and Heckel, A. 2014. Wavelength-Selective Uncaging of Oligonucleotides. Current Protocols in Nucleic Acid Chemistry. 57:6.11:6.11.1–6.11.32.

Author Information

  1. Institute for Organic Chemistry and Chemical Biology, Buchmann Institute for Molecular Life Sciences, Goethe-University Frankfurt, Frankfurt, Germany

Publication History

  1. Published Online: 24 JUN 2014


Caged compounds are light-sensitive molecules with temporarily inactivated biological function. The active compound is released upon irradiation, in which exact spatial and temporal control is accomplished. Beyond this inherently irreversible concept of triggering, the idea of multi-wavelength uncaging provides experiments with more complexity. This unit describes the syntheses of protected nucleoside phosphoramidites of caged dTNpHP [pHP = (p-hydroxyphenacyl)], dTDEACM {DEACM = [(7-diethylaminocoumarin-4-yl)methyl]} or a dCNDBF {NDBF = [1-(3-nitrodibenzofuran-1-yl)ethyl]} modification on the nucleobase, their incorporation in oligonucleotides, characterization, and their wavelength-selective uncaging up to four levels. Curr. Protoc. Nucleic Acid Chem. 57:6.11.1-6.11.32. © 2014 by John Wiley & Sons, Inc.


  • uncaging;
  • photolabile-protecting groups;
  • selective photochemistry;
  • quantum yields