Unit

UNIT 7.2 NMR Determination of Oligonucleotide Structure

  1. Thomas L. James

Published Online: 1 MAY 2001

DOI: 10.1002/0471142700.nc0702s00

Current Protocols in Nucleic Acid Chemistry

Current Protocols in Nucleic Acid Chemistry

How to Cite

James, T. L. 2001. NMR Determination of Oligonucleotide Structure. Current Protocols in Nucleic Acid Chemistry. 00:7.2:7.2.1–7.2.16.

Author Information

  1. University of California, San Francisco, California

Publication History

  1. Published Online: 1 MAY 2001
  2. Published Print: FEB 2000

Abstract

This unit provides an overview of the use of NMR to determine oligonucleotide structure. It covers basic NMR spectral properties, acquisition of interproton distance restraints and torsion angle restraints, structure refinement, assessment of the quality of the structure obtained. Software programs used in the process are also described.