UNIT 7.4 Calorimetry of Nucleic Acids
Published Online: 1 MAY 2001
Copyright © 2003 by John Wiley and Sons, Inc.
Lab Protocol Title
Current Protocols in Nucleic Acid Chemistry
How to Cite
Pilch, D. S. 2001. Calorimetry of Nucleic Acids. Current Protocols in Nucleic Acid Chemistry. 00:7.4:7.4.1–7.4.9.
- Published Online: 1 MAY 2001
- Published Print: FEB 2000
This is not the most recent version of the article. View current version (1 DEC 2015)
This unit describes the application of differential scanning and isothermal titration calorimetry (DSC and ITC) to the study of the thermodynamics of nucleic acid structure. DSC is used to study order-disorder transitions. A single DSC profile provides a wealth of thermodynamic and extrathermodynamic information: transition enthalpy, entropy, free energy, heat capacity, the state of the transition (two-state vs. multistate), and the size of the cooperative unit. ITC is used to study hybridization of nucleic acids at constant temperature. Results can be used to determine the stoichiometry of the association reaction, the enthalpy of association, the equilibrium association constant, and the free energy and entropy of association. A thorough discussion is presented of the details required to obtain meaningful results, as well as relevant methods for analyzing the data produced.