Unit

UNIT 7.6 Methods to Crystallize RNA

  1. Adrian R. Ferré-D'Amaré1,
  2. Jennifer A. Doudna2

Published Online: 1 MAY 2001

DOI: 10.1002/0471142700.nc0706s00

Current Protocols in Nucleic Acid Chemistry

Current Protocols in Nucleic Acid Chemistry

How to Cite

Ferré-D'Amaré, A. R. and Doudna, J. A. 2001. Methods to Crystallize RNA. Current Protocols in Nucleic Acid Chemistry. 1:7.6.1–7.6.13.

Author Information

  1. 1

    Fred Hutchinson Cancer Research Center, Seattle, Washington

  2. 2

    Yale University, New Haven, Connecticut

Publication History

  1. Published Online: 1 MAY 2001
  2. Published Print: FEB 2000

Abstract

Preparation of suitably large and well-ordered single crystals is usually the rate-limiting step in the determination of the three-dimensional structure of RNAs and their complexes with proteins by X-ray crystallography. This unit discusses a variety of experimental considerations for obtaining crystals of RNAs and RNA-protein complexes. Topics include design of crystallizable constructs, screening, and optimization of crystallization conditions.