UNIT 7.6 Methods to Crystallize RNA
Published Online: 1 MAY 2001
Copyright © 2003 by John Wiley and Sons, Inc.
Lab Protocol Title
Current Protocols in Nucleic Acid Chemistry
How to Cite
Ferré-D'Amaré, A. R. and Doudna, J. A. 2001. Methods to Crystallize RNA. Current Protocols in Nucleic Acid Chemistry. 1:7.6.1–7.6.13.
- Published Online: 1 MAY 2001
- Published Print: FEB 2000
Preparation of suitably large and well-ordered single crystals is usually the rate-limiting step in the determination of the three-dimensional structure of RNAs and their complexes with proteins by X-ray crystallography. This unit discusses a variety of experimental considerations for obtaining crystals of RNAs and RNA-protein complexes. Topics include design of crystallizable constructs, screening, and optimization of crystallization conditions.