UNIT 7.12 Biophysical Analysis of Triple-Helix Formation

  1. Alexandre S. Boutorine,
  2. Christophe Escudé

Published Online: 1 JUN 2007

DOI: 10.1002/0471142700.nc0712s29

Current Protocols in Nucleic Acid Chemistry

Current Protocols in Nucleic Acid Chemistry

How to Cite

Boutorine, A. S. and Escudé, C. 2007. Biophysical Analysis of Triple-Helix Formation. Current Protocols in Nucleic Acid Chemistry. 29:7.12:7.12.1–7.12.16.

Author Information

  1. Muséum National d'Histoire Naturelle, Paris, France

Publication History

  1. Published Online: 1 JUN 2007
  2. Published Print: JUN 2007


Two methods for DNA triple-helix analysis are described in this unit: a gel-shift assay based on the slower electrophoretic migration of a triplex in a polyacrylamide gel under nondenaturing conditions, and an optical method in which the thermal denaturation of the triple helix is followed by UV spectrophotometry. Both methods give valuable information on the characteristics of DNA triple-helix formation and triplex stability under different conditions.


  • DNA triple helix;
  • nondenaturing gel electrophoresis;
  • gel-shift assay;
  • thermal denaturation;
  • UV spectrophotometry;
  • melting point