UNIT 8.1 Determination of Binding Mode: Intercalation
Published Online: 1 MAY 2001
Copyright © 2003 by John Wiley and Sons, Inc.
Lab Protocol Title
Current Protocols in Nucleic Acid Chemistry
How to Cite
Dedon, P. C. 2001. Determination of Binding Mode: Intercalation. Current Protocols in Nucleic Acid Chemistry. 00:8.1:8.1.1–8.1.13.
- Published Online: 1 MAY 2001
- Published Print: FEB 2000
A small molecule can be assumed to bind to DNA by intercalation between base pairs if it causes lengthening and unwinding of the DNA helix and undergoes changes in its spectral properties, such as DNA-induced hypochromism and quenching of its UV absorbance. DNA lengthening and unwinding can be determined from the change in viscosity of a solution of linear or plasmid DNA, respectively. Intercalation of a ligand can also be seen as a reduction in the UV/visible absorbance of the intercalator, as well as a shift in the absorbance maximum.