Unit

UNIT 8.1 Determination of Binding Mode: Intercalation

  1. Peter C. Dedon

Published Online: 1 MAY 2001

DOI: 10.1002/0471142700.nc0801s00

Current Protocols in Nucleic Acid Chemistry

Current Protocols in Nucleic Acid Chemistry

How to Cite

Dedon, P. C. 2001. Determination of Binding Mode: Intercalation. Current Protocols in Nucleic Acid Chemistry. 00:8.1:8.1.1–8.1.13.

Author Information

  1. Massachusetts Institute of Technology, Cambridge, Massachusetts

Publication History

  1. Published Online: 1 MAY 2001
  2. Published Print: FEB 2000

Abstract

A small molecule can be assumed to bind to DNA by intercalation between base pairs if it causes lengthening and unwinding of the DNA helix and undergoes changes in its spectral properties, such as DNA-induced hypochromism and quenching of its UV absorbance. DNA lengthening and unwinding can be determined from the change in viscosity of a solution of linear or plasmid DNA, respectively. Intercalation of a ligand can also be seen as a reduction in the UV/visible absorbance of the intercalator, as well as a shift in the absorbance maximum.