Unit

UNIT 8.2 Determination of Binding Thermodynamics

  1. Peter C. Dedon

Published Online: 1 MAY 2001

DOI: 10.1002/0471142700.nc0802s00

Current Protocols in Nucleic Acid Chemistry

Current Protocols in Nucleic Acid Chemistry

How to Cite

Dedon, P. C. 2001. Determination of Binding Thermodynamics. Current Protocols in Nucleic Acid Chemistry. 00:8.2:8.2.1–8.2.8.

Author Information

  1. Massachusetts Institute of Technology, Cambridge, Massachusetts

Publication History

  1. Published Online: 1 MAY 2001
  2. Published Print: FEB 2000

Abstract

This unit serves as a starting point for exploring the thermodynamic properties of interactions between small molecules and DNA. It covers the determination of simple, apparent association/dissociation constants. The concentration of DNA-bound ligand and free ligand are determined and a binding constant is extracted from these data. Data gathering and curve fitting are discussed.