UNIT 8.2 Determination of Binding Thermodynamics
Published Online: 1 MAY 2001
Copyright © 2003 by John Wiley and Sons, Inc.
Lab Protocol Title
Current Protocols in Nucleic Acid Chemistry
How to Cite
Dedon, P. C. 2001. Determination of Binding Thermodynamics. Current Protocols in Nucleic Acid Chemistry. 00:8.2:8.2.1–8.2.8.
- Published Online: 1 MAY 2001
- Published Print: FEB 2000
This unit serves as a starting point for exploring the thermodynamic properties of interactions between small molecules and DNA. It covers the determination of simple, apparent association/dissociation constants. The concentration of DNA-bound ligand and free ligand are determined and a binding constant is extracted from these data. Data gathering and curve fitting are discussed.