Unit

UNIT 8.5 A Fluorescent Intercalator Displacement Assay for Establishing DNA Binding Selectivity and Affinity

  1. Winston C. Tse1,
  2. Dale L. Boger2

Published Online: 1 APR 2005

DOI: 10.1002/0471142700.nc0805s20

Current Protocols in Nucleic Acid Chemistry

Current Protocols in Nucleic Acid Chemistry

How to Cite

Tse, W. C. and Boger, D. L. 2005. A Fluorescent Intercalator Displacement Assay for Establishing DNA Binding Selectivity and Affinity. Current Protocols in Nucleic Acid Chemistry. 20:8.5:8.5.1–8.5.11.

Author Information

  1. 1

    Gilead Sciences, Foster City, California

  2. 2

    The Scripps Research Institute, La Jolla, California

Publication History

  1. Published Online: 1 APR 2005
  2. Published Print: MAR 2005

Abstract

A protocol for a fluorescent intercalator displacement (FID) assay useful for establishing DNA binding selectivity, affinity, stoichiometry, and binding site size, and for distinguishing modes of DNA binding is presented.

Keywords:

  • DNA binding assay;
  • DNA sequence selectivity;
  • intercalator;
  • fluorescence;
  • FID